Composite
Part:BBa_K2139009:Design
Designed by: UBC iGEM 2016 Group: iGEM16_British_Columbia (2016-10-14)
Ptac RBS Endo5a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1062
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 493
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 390
Design Notes
Paenibacillus strain ICGEB2008
Source
It is codon optimized for Caulobacter crescentus, exhibiting a high GC content.